Analysis of microbiota in stable patients with chronic obstructive pulmonary disease.
نویسندگان
چکیده
To identify the bacterial diversity (microbiota) in expectorated sputum, a pyrosequencing method that investigates complex microbial communities of expectorated sputum was done in 19 stable chronic obstructive pulmonary disease patients (mean (SD) FEV1: 47 (18%) of predicted value). Using conventional culture, 3 phyla and 20 bacterial genera were identified, whereas the pyrosequencing approach detected 9 phyla and 43 genera (p < 0.001). In sputum the prevalent genera with pyrosequencing approach were Streptococcus, Actinomyces, Neisseria, Haemophilus, Rothia, Fusobacterium, Gemella, Granulicatella, Porphyromonas, Prevotella and Veillonella. Enterobacteriaceae, detected frequently in conventional culture, were not significantly detected with pyrosequencing methods. In addition, we found that important pathogens such as Haemophilus and Moraxella were detected more frequently with the new genetic procedures. The presence of Enterobacteriaceae is probably overestimated with conventional culture, whereas other difficult cultivable pathogens are underestimated. These studies open a new perspective for evaluating the role of bacterial colonization in chronic obstructive pulmonary disease pathogenesis and progression.
منابع مشابه
The Relationship between Diaphragmatic Movements in Sonographic Assessment and Disease Severity in Patients with Stable Chronic Obstructive Pulmonary Disease (COPD)
Introduction: Pulmonary hyperinflation in patients with chronic obstructive pulmonary disease (COPD) can increase the breathing rate and reduce diaphragmatic movements by pushing the diaphragms downward and limiting their movements; this, in fact, can affect the breathing process. The purpose of this study was to compare diaphragmatic movements in COPD patients and healthy ones and to evaluate ...
متن کاملThe Effect of Motivational Abdominal Breathing Device on Breathing Patterns and Shortness of Breath in Patients with Chronic Obstructive Pulmonary Disease
Background and purpose: Despite the high prevalence of chronic obstructive pulmonary disease, There haven,t been definite curative treatment for the disease and focus of treatment has directed towards pulmonary rehabilitation as an effective non-drug treatments. this study were performed to determine the effect of motivational abdominal breathing device on breathing pattern and dyspnea of chron...
متن کاملEvaluation of Prognostic Factors of Mortality in Patients with Chronic Obstructive Pulmonary Disease
Background and Objective: Chronic Obstructive Pulmonary Disease (COPD) is a chronic obstructive and irreversible disease which has a high mortality and morbidity rate. Systemic inflammation and the thrombotic process can influence the prognosis of these patients. The objectives of this study were to evaluate prognostic effects of CBC indices (WBC, PMN, MPV, RDW), forced expiratory volume-one se...
متن کاملEffect of Pulmonary Rehabilitation Program on Severity of Symptoms in Patients with Chronic Obstructive Pulmonary Disease
800x600 Background and Objectives: Cough and dyspnea are two most common symptoms in chronic obstructive pulmonary disease patients. The aim of this study was to investigate the effect of pulmonary rehabilitation program on severity of symptoms in patients with chronic obstructive pulmonary disease. Materials and Methods: This study was a clinical trial in which 70 patien...
متن کاملDetermination of The Relationship Between Severity of Obstructive Sleep Apnea And Chronic Obstructive Pulmonary Disease
Introduction: Chronic obstructive pulmonary disease (COPD) patients are at increased risk of sleep-disorders. The concomitant occurrence of COPD and obstructive sleep apnea hypopnea syndrome (OSAHS) is named overlap syndrome. This study aimed to evaluate the severity of OSAHS in overlap syndrome patients.Materials & Methods: This cross-sectional study was conducted on adult patients with forced...
متن کاملAssessment of Insomnia Severity in Elderly Patients with Chronic Obstructive Pulmonary Disease in Rasoul Akram Hospital, 2019 Tehran
Background & objectives: Chronic obstructive pulmonary disease (COPD) is one of the most common diseases in the elderly. Elderly people with this disease have many problems, as sleep disorders, that can cause many complications such as falls, depression, memory disorders, irritability, fatigue, difficulty concentrating and dementia. The aim of this study was to determine the severity of insom...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
دوره 123 5 شماره
صفحات -
تاریخ انتشار 2015